Professor Layton and the Spectre's Call will launch across Europe on 25th November, Nintendo has announced.
But European gamers will miss out on 100-hour RPG London Life, an extra mode found in US and Japanese copies of the game.
It features characters from the Layton series and is developed by Brownie Brown, the Nintendo-owned studio behind GBA cult classic Mother 3 - which also never arrived on European shores.
Spectre's Call is the fourth Layton game, but serves, chronologically, as a prequel to the existing DS trilogy.
The series will jump to the 3DS for the next instalment. Mask of Miracles, due 2012 in Europe, launched in Japan earlier this year.
Professor Layton will then appear in a 3DS cross-over game with Capcom's Ace Attorney series.